高通量测序分析Nisin对腹泻小鼠肠道菌群的影响

doi:10.11843/j.issn.0366-6964.2018.09.023

Abstract

Abstract:

In this study, high-throughput sequencing was used to study the effect of Nisin on intestinal microflora in diarrhea mice. The model of diarrhea mice was established by peritoneal perfusion of E. coli O₁, and the best dose of the drug was screened by animal protection test in vivo. The mice were randomly divided into blank control group, negative control group, ampicillin, ciprofloxacin hydrochloride group and Nisin group. Gavage was continued for 15 days, and cecum stool were collected. Five samples were analyzed by high-throughput sequencing. The results showed that:1) E. coli O₁ can duodenal villi in diarrhea mice. Nisin increased the percentage of lymphocytes, to enhance immunity. 2) The best Nisin dose (0.002 g·mL^-1) was screened by mouse protection rate test, the protection rate was 37.5%, lower than that of ampicillin (50%), ciprofloxacin hydrochloride group. 3) Counting of cecal colonies:the number of E. coli and Enterococci in Nisin was lower than that in the negative control group (P<0.05), especially in Nisin group. The number of Lactobacillus in Nisin group was higher than that in other group (P<0.05). 4)The diversity index (ACE=1 417.25, Chao1=1 378.45, Shannon=7.56) in Nisin group was higher than that of negative control group (ACE=969.54, Chao1=340.29, Shannon=6.63). There are significant differences in the flora structure. 5) The Bacteroidetes were the lowest in the Nisin group, with the highest proportion of Firmicutes, Clostridium, Akkermansia and Lactobacillus being higher than Other groups. Therefore, Nisin enhanced the immune function of diarrhea mice, increased the number of beneficial bacteria in the intestine and reduced the number of harmful bacteria; intake of appropriate amount of Nisin can regulate the proportion of intestinal flora.

CLC Number:

S855.12

HE Mei-ling, WANG Chun-jie, JIA Zhi-feng, SI-mu-ji-de, AO-ri-ge-le. The Effect of Nisin on Intestinal Flora in Diarrheal Mice Analyzed by High-throughput Sequencing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 2015-2024.

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The Effect of Nisin on Intestinal Flora in Diarrheal Mice Analyzed by High-throughput Sequencing

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